Electricity Generation by Electrogenic Bacteria using Wastewater as Nutrient

الملخص

This study focuses on finding and describing bacteria from wastewater that can generate electricity, looking at how well they work without oxygen and how they can be used to clean wastewater. Wastewater specimens, collected from diverse locales in Khartoum state, underwent isolation processes employing serial dilution and plating on LB agar medium, resulting in the identification of bacterial isolates including Actinomyces sp, Bacillus sp, Pseudomonas aeruginosa, Staphylococcus sp, Micrococcus sp, Citrobacter sp, Enterobacter sakazakii, and Enterobacter areogenesa. The isolates were authenticated via biochemical methodologies and selective media. Subsequent assessment of the electrogenic potential of these bacteria, both in pure and mixed cultures, occurred within Microbial Fuel Cells (MFCs) utilizing untreated wastewater. Remarkable electricity generation was observed in the mixed culture at 37°C, yielding 1.2 V. Additionally, Actinomyces sp in the pure culture at 30°C demonstrated significant electrochemical output, generating 353 mV after a five-day incubation period. Concurrently, comprehensive analyses of wastewater quality indicators, encompassing total suspended solids and total dissolved solids, revealed substantial reductions, affirming the adept wastewater treatment capabilities of the isolated bacteria. This study underscores the prospect of leveraging wastewater-derived bacteria for electrochemical applications and wastewater treatment, presenting a resource-efficient and sustainable pattern.